Cartilage regeneration in experimental lesion in sheep animal model, by applying autologous bone marrow stem cells expanded ex vivo with BST methodology

Objectives

Study in sheep animal model promoted by the Banc de Sang i Teixits (BST). The objective was to evaluate the behaviour of the infused stem cells in joints previously subjected to articular cartilage and meniscal injury.

Methodology

The study was carried out in the experimental operating room of the Hospital Clínico Veterinario of the Universitat Autònoma de Barcelona with prior approval under the control of the Ethics Committee in Animal and Human Research. Cells were selected and expanded in the Department of Chemical Engineering at the same university.

Results

The results of the study were fully satisfactory: significant differences were evident in the joints that had been treated with expanded mesenchymal stem cells with respect to the untreated (control) joints.

After 6 months, the injured articular cartilage showed recovery with hyaline characteristics and injured meniscus showed a complete regeneration on the free edge.

The effect was considered dose-dependent since the more cells infused, the better regeneration results.


STUDY DESCRIPTION
Status: completed
Sponsor: Banc de Sang i Teixits (BST)
CEEAH: Hospital Clínico Veterinario (UAB)
Investigators: F García, J García, F Gòdia, JJ Cairó, D peris, J Barrachina, R Soler Rich, M Pumarola, M Prades, F soler, Ll Orozco
Participating entities: Banc de Sang i Teixits; Dep, Ingeniería Química (UAB); Hospital Clínico Veterinario (UAB); ITRT-Centro Médico Teknon; Facultad Veterinaria (UAB); EGARSAT.
Funding: BST (Proyecto MEDCEL, Secretaria de Estado de Universidades e Investigación)